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产品信息
CSI 006-01-02
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Dog,
Human,
Marsupial,
Sheep,
Pig,
Rabbit
宿主/亚型
Mouse
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
1E2-E4/Col5
抗原
Acid-digested pepsin soluble dog type V collagen.
偶联物
Unconjugated
形式
Liquid
浓度
1.05 mg/mL
纯化类型
Protein A/G
保存液
PBS, pH 7.4
内含物
15mM sodium azide
保存条件
4° C, store in dark
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_1073787
产品详细信息
CSI 006-01-02 is highly specific for type V collagen. It has been shown to have no cross-reactivity with type I, III and VI collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin). CSI 006-01-02 shows reactivity with human, dog, calf, sheep, pig and kangaroo in type V collagens by ELISA and immunoblotting. CSI 006-01-02 does not show reactivity with mouse, rat, guinea pig and chicken type V collagens by ELISA and immunoblotting.
CSI 006-01-02 has been successfully used in Western blotting, ELISA and Immunohistochemistry procedures. CSI 006-01 has been used succesfully for immunohistology on frozen unfixed sections of human, bovine and dog skin, of foetal bovine cornea, and of new dog tissue associated with a biomaterial implant. If fixation of tissue is required, acetone or ethanol is recommended. CSI 006-01 binds poorly to Collagen V when tested in ELISA with Collagen V coated directly onto the microtiter well.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
靶标信息
COL5A1 encodes an alpha chain for one of the lowabundance fibrillar collagens. Fibrillar collagen molecules aretrimers that can be composed of one or more types of alpha chains.Type V collagen is found in tissues containing type I collagen andappears to regulate the assembly of heterotypic fibers composed ofboth type I and type V collagen. COL5A1 product is closelyrelated to type XI collagen and it is possible that the collagenchains of types V and XI constitute a single collagen type withtissue-specific chain combinations. Mutations in this gene areassociated with Ehlers-Danlos syndrome, types I and II.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AB collagen; alpha-1 type V collagen; COL5; Collagen 5; Collagen alpha-1(V) chain; collagen type 5 alpha 1; collagen, type V, alpha 1; procollagen alpha 1(V); RP11-263F14.1; Type V Collagen
基因别名: COL5A1; EDSC
UniProt ID: (Human) P20908
Entrez Gene ID: (Pig) 397533, (Sheep) 101107777, (Dog) 480684, (Rabbit) 100327268, (Human) 1289
blood vessel development
heart morphogenesis
cell adhesion
cell migration
extracellular matrix organization
collagen fibril organization
collagen catabolic process
collagen biosynthetic process
wound healing, spreading of epidermal cells
tendon development
extracellular fibril organization
skin development
integrin biosynthetic process
eye morphogenesis
regulation of cellular component organization
negative regulation of endodermal cell differentiation
